Overview

  • Product name
  • Description
    Rabbit polyclonal to DUSP27
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 250-282 (EALMTVRKKR AIYPNEGFLK QLRELNEKLM EER) of Human DUSP27 (Q5VZP5).

  • Positive control
    • Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab121372 at 1/100 dilution staining DUSP27 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.

References

ab121372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (mouse muscle C2C12)
Specification
mouse muscle C2C12
Treatment
siRNA transfected
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Mouse Cell lysate - whole cell (Kidney)
Specification
Kidney
Treatment
plasmid transfected
Total protein in input
500 µg
Username

Abcam user community

Verified customer

Submitted Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Sample
Mouse Cell (mouse muscle C2C12)
Specification
mouse muscle C2C12
Permeabilization
Yes - 0.3% triton in PBS
Fixative
Paraformaldehyde
Username

Yanfeng

Verified customer

Submitted Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up