Overview

  • Product nameAnti-DUSP27 antibody
  • Description
    Rabbit polyclonal to DUSP27
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 250-282 (EALMTVRKKR AIYPNEGFLK QLRELNEKLM EER) of Human DUSP27 (Q5VZP5).

  • Positive control
    • Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-DUSP27 antibody images

  • ab121372 at 1/100 dilution staining DUSP27 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.

References for Anti-DUSP27 antibody (ab121372)

ab121372 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 60 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (mouse muscle C2C12)
Specification mouse muscle C2C12
Treatment siRNA transfected
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Mouse Cell lysate - whole cell (Kidney)
Specification Kidney
Treatment plasmid transfected
Total protein in input 500 µg
Username

Abcam user community

Verified customer

Submitted Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Sample Mouse Cell (mouse muscle C2C12)
Specification mouse muscle C2C12
Permeabilization Yes - 0.3% triton in PBS
Fixative Paraformaldehyde
Username

Yanfeng

Verified customer

Submitted Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"