Anti-DUSP3 antibody [EPR5492] (ab125077)

Overview

  • Product nameAnti-DUSP3 antibody [EPR5492]
    See all DUSP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5492] to DUSP3
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human DUSP3.

  • Positive control
    • HeLa, Jurkat, MCF7 and K562 cell lysates; Human ovarian cancer tissue and Mouse kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab125077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionShows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 3 antibody
    • Dual specificity protein phosphatase 3 antibody
    • Dual specificity protein phosphatase VHR antibody
    • DUS3_HUMAN antibody
    • DUSP3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • Vaccinia H1-related phosphatase antibody
    • Vaccinia virus phosphatase VH1 related antibody
    • VHR antibody
    see all

Anti-DUSP3 antibody [EPR5492] images

  • All lanes : Anti-DUSP3 antibody [EPR5492] (ab125077) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 20 kDa
  • ab125077, at 1/100, staining DUSP3 in paraffin-embedded Human ovarian cancer tissue by immunohistochemistry.
  • ab125077, at 1/100, staining DUSP3 in paraffin-embedded Mouse kidney tissue by immunohistochemistry.

References for Anti-DUSP3 antibody [EPR5492] (ab125077)

ab125077 has not yet been referenced specifically in any publications.

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The peptide used to raise the anti-DUSP3 antibody [EPR5492] (ab125077) has been taken from the region ofamino acids 1 - 30 of theN-terminus of human DUSP3/VHR (UniProt: P51452). <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"