Overview

  • Product nameAnti-DUSP5 antibody [2296C4a]
    See all DUSP5 primary antibodies
  • Description
    Mouse monoclonal [2296C4a] to DUSP5
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human DUSP5.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab53217 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • ClonalityMonoclonal
  • Clone number2296C4a
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionDisplays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 5 antibody
    • Dual specificity protein phosphatase 5 antibody
    • Dual specificity protein phosphatase hVH 3 antibody
    • Dual specificity protein phosphatase hVH3 antibody
    • DUS5_HUMAN antibody
    • DUSP 5 antibody
    • DUSP antibody
    • DUSP5 antibody
    • HVH 3 antibody
    • HVH3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH 3 antibody
    • VH1 like phosphatase 3 antibody
    • VH3 antibody
    see all

Anti-DUSP5 antibody [2296C4a] images

References for Anti-DUSP5 antibody [2296C4a] (ab53217)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Vascular Smooth Muscle Cells)
Gel Running Conditions Reduced Denaturing (10 % gel)
Loading amount 20 µg
Treatment Anigotensin ii (10 uM) -/+ shDUSP5
Specification Vascular Smooth Muscle Cells
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"