Overview

  • Product nameAnti-DUSP5 antibody
    See all DUSP5 primary antibodies
  • Description
    Rabbit polyclonal to DUSP5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide corresponding to N terminal residues of rat DUSP5.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by antigen-specific affinity chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 42 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDisplays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
      Contains 1 rhodanese domain.
      Contains 1 tyrosine-protein phosphatase domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dual specificity phosphatase 5 antibody
      • Dual specificity protein phosphatase 5 antibody
      • Dual specificity protein phosphatase hVH 3 antibody
      • Dual specificity protein phosphatase hVH3 antibody
      • DUS5_HUMAN antibody
      • DUSP 5 antibody
      • DUSP antibody
      • DUSP5 antibody
      • HVH 3 antibody
      • HVH3 antibody
      • Serine/threonine specific protein phosphatase antibody
      • VH 3 antibody
      • VH1 like phosphatase 3 antibody
      • VH3 antibody
      see all

    References for Anti-DUSP5 antibody (ab54549)

    ab54549 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Murine embryonic fibroblast)
    Loading amount 10 µg
    Specification Murine embryonic fibroblast
    Treatment Serum starved and stimulated for 2 hours
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    Submitted May 01 2013

    Application Western blot
    Sample Human Tissue lysate - whole (H2009 non small cell lung cancer cell line)
    Loading amount 100 µg
    Specification H2009 non small cell lung cancer cell line
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 14 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"