Overview

  • Product name
  • Description
    Goat polyclonal to DUSP6
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human DUSP6 aa 367-381 (C terminal) (Cysteine residue).
    Sequence:

    PSNQNVYQVDSLQST


    Database link: NP_001937.2

  • Positive control
    • WB: Human, mouse, pig and rat heart lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab166922 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26, 42 kDa).

Target

  • Function
    Inactivates MAP kinases. Has a specificity for the ERK family.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 6 antibody
    • Dual specificity phosphatase 6 isoform a antibody
    • Dual specificity protein phosphatase 6 antibody
    • Dual specificity protein phosphatase PYST1 antibody
    • DUS6_HUMAN antibody
    • DUSP 6 antibody
    • DUSP 6a antibody
    • Dusp6 antibody
    • DUSP6a antibody
    • HH19 antibody
    • MAP kinase phosphatase 3 antibody
    • Mitogen activated protein kinase phosphatase 3 antibody
    • Mitogen-activated protein kinase phosphatase 3 antibody
    • MKP 3 antibody
    • MKP-3 antibody
    • MKP3 antibody
    • PYST 1 antibody
    • PYST1 antibody
    • Serine/threonine specific protein phosphatase antibody
    see all

Images

  • All lanes : Anti-DUSP6 antibody (ab166922) at 0.3 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Mouse heart lysate (in RIPA buffer)
    Lane 3 : Rat heart lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 26, 42 kDa
    Observed band size : 26,40 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

  • Anti-DUSP6 antibody (ab166922) at 0.3 µg/ml + Pig heart lysate (in RIPA buffer) at 0.3 µg
    Developed using the ECL technique

    Predicted band size : 26, 42 kDa
    Observed band size : 26,40 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

References

ab166922 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab166922.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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