Anti-DUSP7 antibody (ab100921)

Overview

  • Product name
  • Description
    Rabbit polyclonal to DUSP7
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant full length Human DUSP7 (NM_001947).

  • Positive control
    • HepG2 cell lysate and Human fetal liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab100921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Regulates the activity of the MAP kinase family in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L although it is unclear whether this is by competing for transcription, translation or activation factors.
  • Tissue specificity
    Expressed at significantly higher levels in malignant hematopoietic cells than in corresponding non-malignant cells.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 7 antibody
    • Dual specificity protein phosphatase 7 antibody
    • Dual specificity protein phosphatase PYST2 antibody
    • DUS7_HUMAN antibody
    • Dusp7 antibody
    • Jnk Stimulatory Phosphatase 1 antibody
    • JSP1 antibody
    • MAP kinase phosphatase X antibody
    • MKPX antibody
    • PYST2 antibody
    see all

Images

  • Anti-DUSP7 antibody (ab100921) at 1/500 dilution + HepG2 Cell Lysate

    Predicted band size : 41 kDa
  • ab100921, at 1/100 dilution, staining cytoplasmic DUSP7 in formalin-fixed, paraffin-embedded Human fetal liver tissue, by Immunohistochemistry.

References

ab100921 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary MEF)
Loading amount
5 µg
Specification
Primary MEF
Treatment
100ngµl TPA for 2 hours
Gel Running Conditions
Reduced Denaturing (4-14% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2012

I just wanted to check in with you regarding the issues that you had been experiencing the DUSP7 and DUSP2 antibodies.

Have you had an opportunity to assemble information about your protocol? We take issues such as these seriously and we guara...

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Thank you for your response.

I am sorry about the issues you have been having. Thank you for bringing this to our attention here and with Jason. Unfortunately the image did not arrive with this. It may be that the file size is too large for o...

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Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products. Often times high background can be reduced by using TBS + 0.1% Tween 20 for your washes or by reducing the concentra...

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