Overview

  • Product nameAnti-DUT antibody
    See all DUT primary antibodies
  • Description
    Rabbit polyclonal to DUT
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 25-44 (NARGTAEGRSRGTLRARPAP) and 113-130 (QLRFARLSEHATAPTRGS) of Human DUT-N.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Please note:
    Quality control data for this antibody: 20 ug of HeLa cell lysate was used in one lane of a mini blot. At a 1 min exposure, two bands are seen (as shown on the datasheet): a predominant band at 21 kDa and a lighter band at ~31 kDa.
    At a 15 min exposure, a 90 kDa band is also observed, along with much lighter bands at 66 and 55 kDa. Therefore please note that additional bands can appear with prolonged exposure, high antibody concentrations or high lysate concentrations, this may mask the specific band.
  • Target

    • FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificityFound in a variety of tissues.
    • PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similaritiesBelongs to the dUTPase family.
    • Post-translational
      modifications
      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localizationMitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody images



    • Predicted band size : 27 kDa


      Western blot detection of DUT-N in HeLa cell lysate using ab13690 at a dilution of 1:1,000.

    References for Anti-DUT antibody (ab13690)

    This product has been referenced in:
    • Luo Y  et al. Uracil incorporation into genomic DNA does not predict toxicity caused by chemotherapeutic inhibition of thymidylate synthase. DNA Repair (Amst) 7:162-9 (2008). WB ; Human . Read more (PubMed: 17942376) »
    • Wu M  et al. dUTP Pyrophosphatase, its appearance in extracellular compartment may serve as a potential biomarker for N-methyl-N'-nitro-N-nitrosoguanidine exposure in mammalian cells. Proteomics 6:3001-7 (2006). WB ; Human . Read more (PubMed: 16619294) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"