Overview

  • Product nameAnti-DUT antibody
    See all DUT primary antibodies
  • Description
    Mouse monoclonal to DUT
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: TDIQIALPSG CYGRVAPRSG LAAKHFIDVG AGVIDEDYRG NVGVVLFNFG KEKFEVKKGD RIAQLICERI FYPEIEEVQA LDDTERGSGG FGSTGKN, corresponding to amino acids 68-164 of Human DUT-N

Properties

Applications

Our Abpromise guarantee covers the use of ab54942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificityFound in a variety of tissues.
    • PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similaritiesBelongs to the dUTPase family.
    • Post-translational
      modifications
      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localizationMitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody images

    • DUT-N antibody (ab54942) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.
    • Western blot against tagged recombinant protein immunogen using ab54942 DUT-N antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
    • ab54942 at 10 ug/ml staining DUT-N in human Hela cells by Immunocytochemistry / Immunofluorescence.

    References for Anti-DUT antibody (ab54942)

    ab54942 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab54942.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"