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Product name Anti-DUT antibody See all DUT primary antibodies
Rabbit polyclonal to DUT
Tested applications Suitable for:
ELISA, WB, IHC-P more details
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
Synthetic peptide: FGKEKFEVKK GDRIAQLICE RIFYPEIEEV QALDDTERGS GGFGSTGK, corresponding to a region within C terminal amino acids 204-253 of Human DUT-N
Jurkat cell lysate and Human heart tissue.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
Use a concentration of 2.5 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Function This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Tissue specificity Found in a variety of tissues.
Pathway Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Sequence similarities Belongs to the dUTPase family.
Post-translational modifications Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
Cellular localization Mitochondrion and Nucleus.
Information by UniProt
Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
Deoxyuridine triphosphatase antibody
Anti-DUT antibody images
Western blot - DUT-N antibody (ab65444)
Anti-DUT antibody (ab65444) at 2.5 µg/ml + Jurkat cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUT-N antibody (ab65444)
ab65444, at 4µg/ml, staining DUT-N in paraffin embedded Myocardial cells of Human heart (indicated by arrows) by Immunohistochemistry tissue.
References for Anti-DUT antibody (ab65444)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"