Overview

  • Product nameAnti-DUT antibody
    See all DUT primary antibodies
  • Description
    Rabbit polyclonal to DUT
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence mapping at the middle region of human DUT-N, identical to the related rat and mouse sequence.

  • Positive control
    • Rat cardiac muscle tissue lysate and rat cardiac muscle tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 1 - 2 µg/ml. Boiling the sections is required.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: ~23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • Tissue specificityFound in a variety of tissues.
    • PathwayPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • Sequence similaritiesBelongs to the dUTPase family.
    • Post-translational
      modifications
      Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • Cellular localizationMitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody images

    • ab65971 at 1µg/ml staining DUT-N in the cytoplasm of paraffin-embedded rat tissue sections (cardiac muscle). (DAB chromogenic reaction)
    • Anti-DUT antibody (ab65971) at 0.5 µg/ml + Rat cardiac muscle tissue lysate

      Predicted band size : 23 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    References for Anti-DUT antibody (ab65971)

    This product has been referenced in:
    • Luo Y  et al. Uracil incorporation into genomic DNA does not predict toxicity caused by chemotherapeutic inhibition of thymidylate synthase. DNA Repair (Amst) 7:162-9 (2008). Read more (PubMed: 17942376) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"