Overview

  • Product name
    Anti-Dux4 antibody [E5-5]
    See all Dux4 primary antibodies
  • Description
    Rabbit monoclonal [E5-5] to Dux4
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    .

  • Positive control
    • WB: Human, mouse and rat testis and mouse and rat brain tissue lysates. HT 1080, PC 3, and HeLa cells lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    E5-5
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Relevance
      DUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • Double homeobox 4 antibody
      • Double homeobox protein 10 antibody
      • Double homeobox protein 4 antibody
      • Double homeobox protein 4/10 antibody
      • DUX10 antibody
      • DUX4L antibody
      • DUX4L1 antibody
      see all

    Images

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : HT-1080 whole cell lysate
      Lane 2 : PC-3 whole cell lysate
      Lane 3 : Saos-2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse testis tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa


      Exposure time : 3 minutes
    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)

      Lane 1 : Human testis lysate
      Lane 2 : HT 1080 cell lysate
      Lane 3 : PC 3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 45 kDa

    References

    This product has been referenced in:
    • Homma S  et al. Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4. Skelet Muscle 6:42 (2016). ICC/IF ; Human . Read more (PubMed: 27906075) »
    • Choi SH  et al. DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes. Nucleic Acids Res 44:5161-73 (2016). IP . Read more (PubMed: 26951377) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Rhabdomyosarcoma cell line)
    Specification
    Rhabdomyosarcoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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