Overview

  • Product nameAnti-Dux4 antibody [E5-5]
    See all Dux4 primary antibodies
  • Description
    Rabbit monoclonal [E5-5] to Dux4
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Dux4.

  • Positive control
    • WB: Human, mouse and rat testis and mouse and rat brain tissue lysates. HT 1080, PC 3, and HeLa cells lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberE5-5
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • RelevanceDUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • Double homeobox protein 10 antibody
      • Double homeobox protein 4 antibody
      • Double homeobox protein 4/10 antibody
      • DUX10 antibody
      • DUX4L1 antibody
      see all

    Anti-Dux4 antibody [E5-5] images

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : HT-1080 whole cell lysate
      Lane 2 : PC-3 whole cell lysate
      Lane 3 : Saos-2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse testis tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa


      Exposure time : 3 minutes
    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)

      Lane 1 : Human testis lysate
      Lane 2 : HT 1080 cell lysate
      Lane 3 : PC 3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 45 kDa

    References for Anti-Dux4 antibody [E5-5] (ab124699)

    This product has been referenced in:
    • Ferri G  et al. Direct interplay between two candidate genes in FSHD muscular dystrophy. Hum Mol Genet 24:1256-66 (2015). ChIP ; Human . Read more (PubMed: 25326393) »
    • Himeda CL  et al. Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene. Mol Cell Biol 34:1942-55 (2014). ICC/IF . Read more (PubMed: 24636994) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Rhabdomyosarcoma cell line)
    Specification Rhabdomyosarcoma cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton X-100 in PBS
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"