Recombinant Anti-Dux4 antibody [E5-5] (ab124699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E5-5] to Dux4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Dux4 antibody [E5-5]
See all Dux4 primary antibodies -
Description
Rabbit monoclonal [E5-5] to Dux4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis tissue lysates, HT-1080, PC-3, Saos-2, HeLa, 293T and A549 whole cell lysates.
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General notes
For details on the development of this clone, please refer to Geng et al. Hybridoma (Larchmt). 2011 Apr; 30(2): 125–130.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E5-5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse testis normal tissue lysate - total protein (ab29289)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Relevance
DUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 100288687 Human
- Omim: 606009 Human
- SwissProt: Q9UBX2 Human
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Alternative names
- Double homeobox 4 antibody
- Double homeobox protein 10 antibody
- Double homeobox protein 4 antibody
see all
Images
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All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : HFF-1 (Human skin fibroblast) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 60 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBSTThe expression profile observed in HFF-1 is consistent with the literature (PMID: 30420784).
Negative control : HFF-1 (PMID: 30420784) -
All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)
Lane 1 : HT1080 (human fibrosarcoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : Saos-2 (human osteosarcoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)
Lane 1 : Human testis lysate
Lane 2 : HT1080 (human fibrosarcoma cell line) cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 45 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab124699 has been referenced in 30 publications.
- Gros M et al. Identification of Serum Interleukin 6 Levels as a Disease Severity Biomarker in Facioscapulohumeral Muscular Dystrophy. J Neuromuscul Dis 9:83-93 (2022). PubMed: 34459413
- Hangul C et al. Analysis of DUX4 Expression in Bone Marrow and Re-Discussion of DUX4 Function in the Health and Disease. Turk Patoloji Derg 38:219-226 (2022). PubMed: 34854471
- Ashoti A et al. Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen. PLoS One 17:e0263262 (2022). PubMed: 35176052
- Masteika IF et al. Downstream events initiated by expression of FSHD-associated DUX4: Studies of nucleocytoplasmic transport, γH2AX accumulation, and Bax/Bak-dependence. Biol Open 11:N/A (2022). PubMed: 35191484
- Vuoristo S et al. DUX4 is a multifunctional factor priming human embryonic genome activation. iScience 25:104137 (2022). PubMed: 35402882