Overview

  • Product name
  • Description
    Rabbit polyclonal to Dynamin 1
  • Specificity
    ab55397 detects endogenous levels of total Dynamin 1 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Dynamin 1, around the phosphorylation site of serine 774 (R-R-S-P-T).

  • Positive control
    • IHC-P: human brain tissue. WB: extracts from mouse brain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab55397 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

Anti-Dynamin 1 antibody images

  • ab55397, at 1/50 dilution, staining Dynamin 1 in human paraffin embedded human brain tissue by Immunohistochemistry in the presence (right image) or absence (left image) of the immunizing peptide.
  • All lanes : Anti-Dynamin 1 antibody (ab55397) at 1/500 dilution

    Lane 1 : Mouse brain extract without immunizing peptide
    Lane 2 : Mouse brain extract with immunizing peptide


    Predicted band size : 97 kDa
    Observed band size : 97 kDa

References for Anti-Dynamin 1 antibody (ab55397)

This product has been referenced in:
  • Tang Z  et al. c-Src and Neural Wiskott-Aldrich Syndrome Protein (N-WASP) Promote Low Oxygen-Induced Accelerated Brain Invasion by Gliomas. PLoS One 8:e75436 (2013). Read more (PubMed: 24069415) »
  • Becker M  et al. Different protein profiles in inferior colliculus and cerebellum: a comparative proteomic study. Neuroscience 154:233-44 (2008). WB ; Rat . Read more (PubMed: 18400408) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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