Overview

  • Product name
    Anti-Dynamin 1 antibody [D5]
    See all Dynamin 1 primary antibodies
  • Description
    Mouse monoclonal [D5] to Dynamin 1
  • Tested applications
    Suitable for: IP, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Cow, Human
  • Immunogen

    Recombinant His-tagged fusion protein, corresponding to amino acids 1-750 of Human Dynamin 1.

  • Positive control
    • This antibody gave a positive signal in Mouse and Rat Brain Tissue Lysates.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab13251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
IHC-P 1/50.

Target

Images

  • All lanes : Anti-Dynamin 1 antibody [D5] (ab13251) at 5 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 104 kDa


    Exposure time : 1 minute
  • Dynamin 1 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Dynamin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13251.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
    Band: 104kDa; Dynamin 1

References

This product has been referenced in:
  • Ku YC  et al. The transcriptome of utricle hair cell regeneration in the avian inner ear. J Neurosci 34:3523-35 (2014). Read more (PubMed: 24599453) »
  • Nath S  et al. A huntingtin-mediated fast stress response halting endosomal trafficking is defective in Huntington's disease. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 25205111) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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