Overview

  • Product name
    Anti-Dynamin 1 antibody [EP772Y]
    See all Dynamin 1 primary antibodies
  • Description
    Rabbit monoclonal [EP772Y] to Dynamin 1
  • Specificity
    This antibody detects both phosphorylated and unphosphorylated Dynamin 1.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues on human Dynamin 1.

  • Positive control
    • WB: 3T3 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IP 1/50.
ICC/IF 1/50.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-Fr or IHC-P.
  • Target

    • Function
      Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
    • Sequence similarities
      Belongs to the dynamin family.
      Contains 1 GED domain.
      Contains 1 PH domain.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
    • Information by UniProt
    • Database links
    • Alternative names
      • B dynamin antibody
      • D100 antibody
      • DNM 1 antibody
      • DNM antibody
      • DNM1 antibody
      • DYN1_HUMAN antibody
      • Dynamin antibody
      • Dynamin-1 antibody
      • Dynamin1 antibody
      see all

    Images

    • Anti-Dynamin 1 antibody [EP772Y] (ab52852) at 1/20000 dilution + 3T3 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 100 kDa

    References

    This product has been referenced in:
    • Reis CR  et al. Crosstalk between Akt/GSK3ß signaling and dynamin-1 regulates clathrin-mediated endocytosis. EMBO J 34:2132-46 (2015). Read more (PubMed: 26139537) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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