Overview

  • Product name
    Anti-Dynamin 1 antibody [EP801Y]
    See all Dynamin 1 primary antibodies
  • Description
    Rabbit monoclonal [EP801Y] to Dynamin 1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus of human dynamin-1.

  • Positive control
    • 3T3 cell lysate, human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Anti-Dynamin 1 antibody (Alexa Fluor® 488) [EP801Y] (ab206051)

    Anti-Dynamin 1 antibody [EP801Y] (ab52611)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300.

For unpurified, use 1/50.

WB 1/2000. Predicted molecular weight: 97 kDa.
IHC-P 1/50.

Target

Anti-Dynamin 1 antibody [EP801Y] images

  • All lanes : Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of Neuro-2a cells with purified ab52611 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52611 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (unpurified) + 3T3 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Unpurified ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.

    Ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.

References for Anti-Dynamin 1 antibody [EP801Y] (ab52611)

This product has been referenced in:
  • Gowrishankar S  et al. Massive accumulation of luminal protease-deficient axonal lysosomes at Alzheimer's disease amyloid plaques. Proc Natl Acad Sci U S A 112:E3699-708 (2015). Read more (PubMed: 26124111) »
  • Tian X  et al. Podocyte-associated talin1 is critical for glomerular filtration barrier maintenance. J Clin Invest 124:1098-113 (2014). Mouse . Read more (PubMed: 24531545) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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