Overview

  • Product name
    Anti-Dynamin 1 (phospho S774) antibody
    See all Dynamin 1 primary antibodies
  • Description
    Rabbit polyclonal to Dynamin 1 (phospho S774)
  • Specificity
    This antibody detects endogenous levels of Dynamin 1 only when phosphorylated at serine 774.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Dynamin 1 around the phosphorylation site of serine 774 (R-R-SP-P-T).

  • Positive control
    • Mouse brain extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted mouse molecular weight: 98 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-Dynamin 1 (phospho S774) antibody (ab55324) at 1/500 dilution

      Lane 1 : Mouse brain extract
      Lane 2 : Mouse brain extract treated with the immunising phosphopeptide.


      Predicted band size : 98 kDa
      Observed band size : 98 kDa

    References

    This product has been referenced in:
    • Reis CR  et al. Crosstalk between Akt/GSK3ß signaling and dynamin-1 regulates clathrin-mediated endocytosis. EMBO J 34:2132-46 (2015). Read more (PubMed: 26139537) »
    • Mori H  et al. Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin ß1. Development 140:343-52 (2013). WB ; Mouse . Read more (PubMed: 23250208) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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