The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted mouse molecular weight: 98 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMicrotubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
Sequence similaritiesBelongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.
Western blot - Dynamin 1 (phospho S774) antibody (ab55324)
All lanes : Anti-Dynamin 1 (phospho S774) antibody (ab55324) at 1/500 dilution
Lane 1 : Mouse brain extract Lane 2 : Mouse brain extract treated with the immunising phosphopeptide.
Predicted band size : 98 kDa Observed band size : 98 kDa
References for Anti-Dynamin 1 (phospho S774) antibody (ab55324)
This product has been referenced in:
Mori H et al. Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin ß1. Development140:343-52 (2013).
Read more (PubMed: 23250208) »
Martins-de-Souza D et al. Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function. Eur Arch Psychiatry Clin Neurosci262:657-66 (2012).
Read more (PubMed: 22350622) »