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Our Abpromise guarantee covers the use of ab3457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Can be blocked with Dynamin 2 peptide (ab4985).|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||1/300. PubMed: 17170215|
|IHC-P||Use a concentration of 1 µg/ml.|
Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).
The nuclear counterstain is DAPI.
Western blot detection of Dynamin 2 on mouse testes homogenate using ab3457.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"