• FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab3458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Dynamin 3 peptide (ab4986). By Western blot, this antibody detects an ~100 kDa protein representing Dynamin 3 from HeLa cell lysate.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionMicrotubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • Sequence similaritiesBelongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • Cellular localizationCytoplasm. Cytoplasm, cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • testicular dynamin antibody
    • testicular antibody
    • DNM 3 antibody
    • DNM3 antibody
    • DNM3 protein antibody
    • Dyn3 antibody
    • DYN3_HUMAN antibody
    • Dyna III antibody
    • DynaIII antibody
    • dynamin 2 antibody
    • Dynamin antibody
    • Dynamin family member antibody
    • Dynamin testicular antibody
    • Dynamin-3 antibody
    • Dynamin3 antibody
    • EC antibody
    • KIAA0820 antibody
    • MGC70433 antibody
    • T dynamin antibody
    • T-dynamin antibody
    see all

Anti-Dynamin 3 antibody images

  • Western blot of Dynamin 3 on rat liver extract using ab3458.

  • ab3458 (4µg/ml) staining Dynamin 3 in human brain cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the white matter region
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab3458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Dynamin 3 antibody (ab3458)

This product has been referenced in:
  • Hyndman KA  et al. Dynamin activates NO production in rat renal inner medullary collecting ducts via protein-protein interaction with NOS1. Am J Physiol Renal Physiol 301:F118-24 (2011). ICC/IF ; Rat . Read more (PubMed: 21490139) »
  • Wang W  et al. Distinct Functional Effects for Dynamin 3 During Megakaryocytopoiesis. Stem Cells Dev : (2011). ICC/IF . Read more (PubMed: 21671749) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (testis tissue)
Specification testis tissue
Fixative Paraformaldehyde
Permeabilization Yes - cold acetone
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Wayne Vogl

Verified customer

Submitted Jan 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK-293T)
Loading amount 20 µg
Specification HEK-293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 13 2007