Dynamin Inhibitors: Iminodyn™ Series Kit (ab120475)

Overview

  • Product nameDynamin Inhibitors: Iminodyn™ Series Kit
  • SpecificityConvenient kit containing dynamin inhibitor Iminodyn™ 22 (ab120461; 1 mg) and Iminodyn™ 17 (ab120462; 1 mg) from the Iminodyn chemical series. Iminodyn™ 22 ab120461 targets dynamin at the GTPase Allosteric Site (GAS) domain. It potently inhibits both dynamin I and II in enzymatic assays and is a potent inhibitor of receptor-mediated endocytosis in cells. Iminodyn™ 17 inhibits dynamin I and II in enzymatic assays, but does not affect dynamin mediated endocytosis in cells. It can therefore be used as a negative control for cell based assays.
  • Product overview

    IminodynTM chemical series collection 

    Convenient kit containing dynamin inhibitor Iminodyn™ 22 (ab120461; 1 mg) and Iminodyn™ 17 (ab120462; 1 mg) from the Iminodyn chemical series. Iminodyn™ 22 (ab120461) targets dynamin at the GTPase Allosteric Site (GAS) domain. It potently inhibits both dynamin I and II in enzymatic assays and is a potent inhibitor of receptor-mediated endocytosis in cells. Iminodyn™ 17 inhibits dynamin I and II in enzymatic assays, but does not affect dynamin mediated endocytosis in cells. It can therefore be used as a negative control for cell based assays. 

    Target

    Description 

    GTPase Allosteric Site 

    Iminodyn-22™:  

    Potent, broad spectrum dynamin inhibitor 

    GTPase Allosteric Site 

    Iminodyn-17™:  

    Negative control for Iminodyn-22™ 

  • Notes

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Iminodyn™ ia a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Iminodyn™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab120475 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Dynamin Inhibitors: Iminodyn™ Series Kit images

    • ab66705 staining PAI1 in HeLa cells treated with iminodyn-22™ (ab120461), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of iminodyn-22™, as described in literature.
      The cells were incubated at 37°C for 48h in media containing different concentrations of ab120461 (Iminodyn-22™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Dynamin Inhibitors: Iminodyn™ Series Kit (ab120475)

    This product has been referenced in:

    See all 2 Publications for this product

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    Thank you for contacting us.

    As we do not undertake our own research we are only able to provide information available in the current literature. Von Kleist et al., (2011) found no cytotoxicity in HeLa cells after 8, 20 or 72 hours and concl...

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    I apologize but we only have full sizes available for each of our products, so we are unable to send small samples. We do, however, fully guarantee each of these inhibitors to meet the specifica...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"