Overview

  • Product name
  • Description
    Rabbit polyclonal to Dynamitin
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Dynamitin (NP_006391.1).

  • Positive control
    • Jurkat, 293T, HeLa and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab124285 was affinity purified using an epitope specific to Dynamitin immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
  • Sequence similarities
    Belongs to the dynactin subunit 2 family.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 50 kD dynein associated polypeptide antibody
    • 50 kDa dynein associated polypeptide antibody
    • 50 kDa dynein-associated polypeptide antibody
    • DCTN-50 antibody
    • DCTN2 antibody
    • DCTN2_HUMAN antibody
    • DCTN50 antibody
    • dynactin 2 (p50) antibody
    • dynactin 2 antibody
    • dynactin complex 50 kD subunit antibody
    • Dynactin complex 50 kDa subunit antibody
    • Dynactin subunit 2 antibody
    • DYNAMITIN antibody
    • p50 dynamitin antibody
    • RBP50 antibody
    see all

Images

  • All lanes : Anti-Dynamitin antibody (ab124285) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Exposure time : 30 seconds
  • ab124285 at 1 µg/ml staining Dynamitin in Jurkat whole cell lysate immunoprecipitated using ab124285 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 44 kDa. Exposure time: 3 seconds

References

ab124285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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