The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
Use at 2-10 µg/mg of lysate.
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
All lanes : Anti-Dynamitin antibody (ab124285) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
ab124285 at 1 µg/ml staining Dynamitin in Jurkat whole cell lysate immunoprecipitated using ab124285 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 44 kDa.
Exposure time: 3 seconds