Anti-p50 dynamitin antibody (ab228704)
Key features and details
- Rabbit polyclonal to p50 dynamitin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p50 dynamitin antibody
See all p50 dynamitin primary antibodies -
Description
Rabbit polyclonal to p50 dynamitin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee -
Immunogen
Recombinant fragment within Human p50 dynamitin (internal sequence). The exact sequence is proprietary.
Database link: Q13561 -
Positive control
- WB: MCF7 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. -
Sequence similarities
Belongs to the dynactin subunit 2 family. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10540 Human
- Entrez Gene: 69654 Mouse
- Entrez Gene: 299850 Rat
- Omim: 607376 Human
- SwissProt: Q3ZCF0 Cow
- SwissProt: Q13561 Human
- SwissProt: Q99KJ8 Mouse
- SwissProt: Q6AYH5 Rat
see all -
Alternative names
- 50 kD dynein associated polypeptide antibody
- 50 kDa dynein associated polypeptide antibody
- 50 kDa dynein-associated polypeptide antibody
see all
Images
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Anti-p50 dynamitin antibody (ab228704) at 1/3000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 44 kDa10% SDS-PAGE
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Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for p50 dynamitin (green) using ab228704 at a 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Paraffin-embedded human breast cancer tissue stained for p50 dynamitin with ab228704 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228704 has not yet been referenced specifically in any publications.