The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
ab53714 might be unsuitable for ICC/IF - please see abreview.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionModulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Sequence similaritiesBelongs to the dynactin subunit 2 family.