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Recombinant fragment: DADTQSKVHQ LYETIQRWSP IASTLPELVQ RLVTIKQLHE QAMQFGQLLT HLDTTQQMIA NSLKDNTTLL TQVQTTMREN LATVEGNFAS IDERMKKLGK , corresponding to amino acids 302-401 of Human Dynamitin
Our Abpromise guarantee covers the use of ab56687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
This image is courtesy of an anonymous AbreviewBlocking Step : 5% Milk for 1 hour at 4°C
IHC image of Dynamitin staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56687, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.