Anti-DYNC1I2 antibody - C-terminal (ab192920)

Overview

  • Product name
    Anti-DYNC1I2 antibody - C-terminal
    See all DYNC1I2 primary antibodies
  • Description
    Rabbit polyclonal to DYNC1I2 - C-terminal
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human DYNC1I2 aa 588-638 (C terminal). The exact sequence is proprietary. NP_001369.1.
    Sequence:

    GDSEGQIVIYDVG EQIAVPRNDE WARFGRTLAE INANRADAEE EAATRIPA


    Database link: Q13409

  • Positive control
    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab192920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.

Target

  • Function
    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
  • Sequence similarities
    Belongs to the dynein intermediate chain family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic dynein 1 intermediate chain 2 antibody
    • Cytoplasmic dynein intermediate chain 2 antibody
    • cytosolic antibody
    • DC1I2_HUMAN antibody
    • DH IC 2 antibody
    • DH IC-2 antibody
    • DNCI2 antibody
    • DNCIC2 antibody
    • Dync1i2 antibody
    • Dynein intermediate chain 2 antibody
    • Dynein intermediate chain 2, cytosolic antibody
    • Dynein, cytoplasmic, intermediate polypeptide 2 antibody
    • IC2 antibody
    see all

Images

  • Detection of DYNC1I2 by Western Blot of Immunprecipitate.
    Lane 1: ab192920 at 1 µg/ml labeling DYNC1I2 in 293T whole cell lysate immunoprecipitated using ab192920 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG.
    Detection: Chemiluminescence with exposure time of 3 seconds.

  • All lanes : Anti-DYNC1I2 antibody - C-terminal (ab192920) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 71 kDa


    Exposure time : 30 seconds

References

ab192920 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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