Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
Belongs to the dynein intermediate chain family. Contains 7 WD repeats.
Phosphorylated upon DNA damage, probably by ATM or ATR. The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
Detection of DYNC1I2 by Western Blot of Immunprecipitate. Lane 1: ab192920 at 1 µg/ml labeling DYNC1I2 in 293T whole cell lysate immunoprecipitated using ab192920 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG. Detection: Chemiluminescence with exposure time of 3 seconds.
Western blot - Anti-DYNC1I2 antibody - C-terminal (ab192920)
All lanes : Anti-DYNC1I2 antibody - C-terminal (ab192920) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK1 whole cell lysate Lane 5 : NIH 3T3 whole cell lysate