Recombinant
RabMAb

Anti-DYNC1I2 antibody [EPR11242] (ab167399)

Overview

  • Product name
    Anti-DYNC1I2 antibody [EPR11242]
    See all DYNC1I2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11242] to DYNC1I2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human DYNC1I2.

  • Positive control
    • Human fetal brain, SH-SY5Y, HeLa, and 293T lysates; Human colon and Human kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
    • Sequence similarities
      Belongs to the dynein intermediate chain family.
      Contains 7 WD repeats.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR. The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytoplasmic dynein 1 intermediate chain 2 antibody
      • Cytoplasmic dynein intermediate chain 2 antibody
      • cytosolic antibody
      • DC1I2_HUMAN antibody
      • DH IC 2 antibody
      • DH IC-2 antibody
      • DNCI2 antibody
      • DNCIC2 antibody
      • Dync1i2 antibody
      • Dynein intermediate chain 2 antibody
      • Dynein intermediate chain 2, cytosolic antibody
      • Dynein, cytoplasmic, intermediate polypeptide 2 antibody
      • IC2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DYNC1I2 with ab167399 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DYNC1I2 with ab167399 at 1/50 dilution.
    • All lanes : Anti-DYNC1I2 antibody [EPR11242] (ab167399) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : HeLa lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 71 kDa

    • Detection of DYNC1I2 by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using ab167399 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab167399 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As



    I have aligned and blasted the immunogen sequence of ab167399 for you and attached the blast results for you in formation.

    ab167399 shows 100% homology with Q13409 isoform 1 and Q13409-5,

    95.2% with Q13409-2,

    94.6% ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up