Overview

  • Product name
  • Description
    Rabbit polyclonal to DYNC1LI2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide within Human DYNC1LI2 aa 159-188 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_006132.1.
    Database link: O43237

  • Positive control
    • NCI-H460 cell lysate. Human cervical carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.9% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174895 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.
  • Sequence similarities
    Belongs to the dynein light intermediate chain family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic dynein 1 light intermediate chain 2 antibody
    • cytosolic antibody
    • DC1L2_HUMAN antibody
    • DNCLI2 antibody
    • Dync1li2 antibody
    • Dynein cytoplasmic 1 light intermediate chain 2 antibody
    • Dynein light intermediate chain 2 antibody
    • dynein light intermediate chain 2 cytosolic antibody
    • dynein, cytoplasmic, light intermediate polypeptide 2 antibody
    • LIC-2 antibody
    • LIC2 antibody
    • LIC53/55 antibody
    • OTTHUMP00000174820 antibody
    see all

Images

  • Anti-DYNC1LI2 antibody (ab174895) at 1/100 dilution + NCI-H460 cell lysate at 35 µg

    Predicted band size : 54 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human cervix carcinoma labeling DYNC1L12 with ab174895 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:
  • Mahale S  et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep 6:22 (2016). Read more (PubMed: 28003657) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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