Overview

  • Product nameAnti-DYNC2H1 antibody
  • Description
    Rabbit polyclonal to DYNC2H1
  • Specificityab122525 shows specific reactivity against DYNC2H1.
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 3954-4051 (DVFNQRNKKS IFPYSVSLPQ SCSILDYRAV IEKIPEDDKP SFFGLPANIA RSSQRMISSQ VISQLRILGR SITAGSKFDR EIWSNELSPV LNLWKKLN) of Human DYNC2H1.

  • Positive control
    • Human small intestine.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells.
  • Involvement in diseaseDefects in DYNC2H1 are the cause of asphyxiating thoracic dystrophy type 3 (ATD3) [MIM:613091]. ATD3 is an autosomal recessive osteochondrodysplasia which often leads to death in infancy because of a severely constricted thoracic cage and respiratory insufficiency.
    Defects in DYNC2H1 are the cause of short rib-polydactyly syndrome type 3 (SRPS3) [MIM:263510]; also called Verma-Naumoff syndrome. A lethal skeletal dysplasia characterized by markedly short ribs, short limbs, polydactyly, narrow thorax, and multiple anomalies of major organs, including heart, intestines, genitalia, kidney, liver, and pancreas.
  • Sequence similaritiesBelongs to the dynein heavy chain family.
  • Cellular localizationCytoplasm > cytoskeleton > cilium axoneme. Cell membrane. Cytoplasm. Localizes to the apical cytoplasm (By similarity). According to PubMed:8666668, it localizes to Golgi apparatus, cytoplasmic vesicle and endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic dynein 2 heavy chain 1 antibody
    • Cytoplasmic dynein 2 heavy chain antibody
    • DYHC2_HUMAN antibody
    • DYNC2H1 antibody
    • Dynein cytoplasmic heavy chain 2 antibody
    • Dynein heavy chain 11 antibody
    • Dynein heavy chain isotype 1B antibody
    • hDHC11 antibody
    see all

Anti-DYNC2H1 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab122525 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122525, at a 1/100 dilution, staining DYNC2H1 in paraffin embedded Human small intestine tissue by Immunohistochemistry.

References for Anti-DYNC2H1 antibody (ab122525)

ab122525 has not yet been referenced specifically in any publications.

Product Wall



These antibodies have not been tested specifically against the other form of dynein, however we have aligned the immunogen sequences against the amino acid sequences of each dynein, and there is no significant similarity to the alternative fo...

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