The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
Anti-Dynein antibody (ab75214) at 1 µg/ml + fetal heart lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 11 kDa Observed band size : 11 kDa Additional bands at : 31 kDa,40 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Dynein antibody (ab75214)
All lanes : Anti-Dynein antibody (ab75214) at 1 µg/ml
Lane 1 : Marker Lane 2 : Zebrafish brain homogenate at 20 µg Lane 3 : Zebrafish heart homogenate at 20 µg Lane 4 : Zebrafish liver homogenate at 20 µg Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Secondary Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution Developed using the ECL technique