Anti-Dynein intermediate chain 1 antibody [74.1] (ab23905)

Overview

  • Product nameAnti-Dynein intermediate chain 1 antibody [74.1]
    See all Dynein intermediate chain 1 primary antibodies
  • Description
    Mouse monoclonal [74.1] to Dynein intermediate chain 1
  • SpecificityThis antibody reacts with all known forms of the 74,000 Dalton intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antibody will cleanly immunoprecipitate the entire dynein complex from TX-100 or NP-40 lysates (including the 530 kD heavy chain, the light intermediate chains, and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates).
  • Tested applicationsSuitable for: IHC (PFA fixed), ICC/IF, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Human, Pig, Xenopus laevis, Drosophila melanogaster
    Predicted to work with: A wide range of mammals
  • Immunogen

    Full length native protein: Purified bovine brain cytoplasmic dynein.

  • Positive control
    • HeLa cell lysate for Western blot.
  • General notes


Properties

Applications

Our Abpromise guarantee covers the use of ab23905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration. PubMed: 24803657
ICC/IF Use a concentration of 2 - 4 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPart of the dynein complex of respiratory cilia.
  • Involvement in diseaseDefects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
    Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
  • Sequence similaritiesBelongs to the dynein intermediate chain family.
    Contains 5 WD repeats.
  • Cellular localizationCytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • Axonemal dynein intermediate chain 1 antibody
    • Axonemal dynein intermediate chain 2 antibody
    • CILD 1 antibody
    • CILD1 antibody
    • Cytoplasmic dynein 1 intermediate chain 1 antibody
    • Cytoplasmic dynein 1 intermediate chain 2 antibody
    • Cytoplasmic dynein intermediate chain 1 antibody
    • Cytoplasmic dynein intermediate chain 2 antibody
    • DH IC 1 antibody
    • DH IC 2 antibody
    • DIC1 antibody
    • DNAI 1 antibody
    • DNAI 2 antibody
    • DNAI1 antibody
    • DNAI1_HUMAN antibody
    • DNAI2 antibody
    • DNCI 2 antibody
    • DNCI1 antibody
    • DNCI2 antibody
    • DNCIC 1 antibody
    • DNCIC 2 antibody
    • DNCIC1 antibody
    • DNCIC2 antibody
    • DYNC1I1 antibody
    • DYNC1I2 antibody
    • Dynein axonemal intermediate chain 1 antibody
    • Dynein axonemal intermediate polypeptide 1 antibody
    • Dynein axonemal intermediate polypeptide 2 antibody
    • Dynein cytoplasmic intermediate polypeptide 1 antibody
    • Dynein cytoplasmic intermediate polypeptide 2 antibody
    • Dynein intermediate chain 1 axonemal antibody
    • Dynein intermediate chain 1 cytosolic antibody
    • Dynein intermediate chain 1, axonemal antibody
    • Dynein intermediate chain 2 axonemal antibody
    • Dynein intermediate chain 2 cytosolic antibody
    • Dynein intermediate chain DNAI1 antibody
    • IC74 antibody
    • ICS antibody
    • ICS1 antibody
    • Immotile cilia syndrome 1 antibody
    • MGC26204 antibody
    • PCD antibody
    see all

Anti-Dynein intermediate chain 1 antibody [74.1] images

  • Immunofluorescent analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (Proab23905) at a dilution of 1:20 overnight at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of Dynein in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (ab23905) at a dilution of 1:20 overnight at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of Dynein in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (ab23905) at a dilution of 1:20 overnight at 4 oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Flow cytometry analysis of Dynein intermediate chain 1 showing positive staining in the cytoplasm of U87-MG cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab23905 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
  • Flow cytometry analysis of Dynein intermediate chain 1 showing strong positive staining in the cytoplasm of Hela cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab23905 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
  • Flow cytometry analysis of Dynein intermediate chain 1 showing positive staining in the cytoplasm of C6 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab23905 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.


  • Predicted band size : 73 kDa
    Western blot detection of Dynein intermediate chain 1 in HeLa cell lysate using ab23905.
  • Anti-Dynein intermediate chain 1 antibody [74.1] (ab23905) at 1/1000 dilution + Rat Sciatic nerve whole tissue lysate at 60 µg

    Secondary
    HRP-conjugated Goat anti-mouse IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 73 kDa

    This image is courtesy of an anonymous Abreview

    Gel: 10% acryl amid
    Blocking Step: 5% Milk for 30 minutes at 25

    See Abreview

  • Overlay histogram showing DU145 cells stained with ab23905 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23905, 1µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Dynein intermediate chain 1 antibody [74.1] (ab23905)

This product has been referenced in:
  • Jayappa KD  et al. Human immunodeficiency virus type 1 employs the cellular dynein light chain 1 protein for reverse transcription through interaction with its integrase protein. J Virol 89:3497-511 (2015). WB, IP, Flow Cyt ; Human . Read more (PubMed: 25568209) »
  • Turgay Y  et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol 204:1099-109 (2014). Human . Read more (PubMed: 24662567) »

See all 13 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Raji)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Specification Raji
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Xiao Qing Lu

Verified customer

Submitted Jan 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 25000 cells
Specification HeLa
Treatment 2,5mM Thymidine or 50 ng/ml nocodazole for 16hrs and released as indicated
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step 2%milk, 1%BSA in PBS-Tween-20 (0,1%) as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (Sciatic nerve)
Total protein in input 40 µg
Specification Sciatic nerve
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Sciatic nerve)
Loading amount 60 µg
Specification Sciatic nerve
Gel Running Conditions Reduced Denaturing (10% acryl amid)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 11 2010

Application Western blot
Sample Mouse Cell lysate - other (Primary cortical neuron)
Loading amount 10 µg
Specification Primary cortical neuron
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jan 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"