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Overview

  • Product nameDyngo-4a™
  • Description
    Novel, highly potent dynamin inhibitor. Dynasore (ab120192) analog.
  • Biological descriptionNovel, highly potent dynamin inhibitor (IC50 values are 380 nM and 2.6 μM at dynamin I and II respectively). Dynasore (ab120192) analog. Prevents botulin neurotoxin A (BoNTs) uptake and internalisation, and prevents SNAP25 cleavage. Inhibits BoNTs induced paralysis and delays botulism onset.
  • General notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dyngo4A™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

    • Properties

  • Chemical name3-Hydroxy-N'-[(2,4,5-trihydroxyphenyl)methylidene]naphthalene-2-carbohydrazide
  • Molecular Weight338.31
  • Chemical structureChemical Structure
  • Molecular formulaC18H14N2O5
  • Storage instructionsStore at +4°C (desiccating conditions).
  • Solubility overviewSoluble in DMSO
  • Handling

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

  • Source

    Synthetic

  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab120689 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent concentration.

    Dyngo-4a™ images

    • Functional Studies - Dyngo-4a™ (ab120689)
      Functional Studies - Dyngo-4a™ (ab120689)

      ab66705 staining PAI1 in HeLa cells treated with dyngo-4a™(ab120689), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dyngo-4a™, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dyngo-4a™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Dyngo-4a™ (ab120689)

    This product has been referenced in:
    • Harper CB  et al. Targeting membrane trafficking in infection prophylaxis: dynamin inhibitors. Trends Cell Biol 23:90-101 (2013). Read more (PubMed: 23164733) »
    • Harper CB  et al. Dynamin inhibition blocks botulinum neurotoxin type A endocytosis in neurons and delays botulism. J Biol Chem 286:35966-76 (2011). Read more (PubMed: 21832053) »

    See all 3 Publications for this product

    Publishing research using ab120689 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab120689

    Displaying 1 - 4 of 4 results for Q&A

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    Inquiry: Hi, I was wondering if Dyngo 4a is a reversible inhibitor of dynamin (another inhibitor you sell, Pitstop 2, is reversible upon washing). Also, does serum in the medium inhibit the effects of Dyngo?

    Thank you for contacting us.

    Unfortunately we do not test the biological activity of our compounds in-house but I have consulted the literature for these types of questions. So far there have been 3 publications using this product:


    ...

    Read More

    Dear Sir/Madam

    I have purchased dynasore and dyngo 4a from you along with the dynamin toolkit containing iminodyn, dynole and MAB series compounds. The dynamin tool kit compounds contain infomation regarding dynamin binding sites but this infor...

    Read More

    Thank you for your enquiry.

    Please find information about the biological activity of these compounds below. We also have specific references listed on the datasheet that will provide more information. Reviewing the online literature I have also...

    Read More

    On the Dynamin inhibitors summary table (http://www.ascentscientific.com/Signalling/Endocytosis-&-exocytosis/Dynamin/c/1136), the IC50 of Dyngo-4a for Receptor Mediated Endocytosis Inhibition is >300 µm.
    In some publications a total inhi...

    Read More

    Merci de nous avoir contactés.

    Mes collègues d'Ascent m'ont informé qu'une erreur s'est glissée dans le tableau que vous avez mentionné. La colonne 'receptor mediated endocytosis' ne devrait pas comporter de va...

    Read More

    Phone call requesting advice on the solubility of Dyngo-4a. Have made a stock solution of 30 mM which has then been diluted to 1/1000 but the compound is precipitating out.

    Thank you for contacting us.

    Our chemistry team have previously investigated the solubility of Dyngo-4a™. They found that it is soluble to 100 mM in DMSO. They also found that it was possible to achieve a concentration of 0.03 mM in 1 % DMSO/wa...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"