Overview

  • Product nameAnti-DYNLT3 antibody
  • Description
    Rabbit polyclonal to DYNLT3
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    antigen sequence: DEVGFNAEEA HNIVKECVDG VLGGEDYNHN NINQWTASIV EQSLTHLVKL GKAYKYIVTC AVVQKSAYGF HTASSCFWDT TSDGTCTVRW E, corresponding to amino acids 9-99 of Human DYNLT3.

  • Positive control
    • U 251 MG cells; RT 4, U 251 MG, Human liver and Human tonsil lysates; Human cerebral cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

Target

  • FunctionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Probably binds BUB3 as part of transport cargo. Required for the efficient progression through mitosis.
  • Sequence similaritiesBelongs to the dynein light chain Tctex-type family.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Chromosome > centromere > kinetochore. Colocalizes with BUB3 at kinetochores specifically during prometaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310075M16Rik antibody
    • AU042796 antibody
    • DYLT3_HUMAN antibody
    • Dynein light chain Tctex-type 3 antibody
    • DYNLT3 antibody
    • Protein 91/23 antibody
    • RP23-256J17.2 antibody
    • RP3 antibody
    • T-complex-associated testis-expressed 1-like antibody
    • Tcte1l antibody
    • TCTEX1L antibody
    see all

Anti-DYNLT3 antibody images

  • ab121209 at 1/750 dilution staining DYNLT3 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • All lanes : Anti-DYNLT3 antibody (ab121209) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab121209 at 4 µg/ml staining DYNLT3 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

References for Anti-DYNLT3 antibody (ab121209)

This product has been referenced in:
  • Chu Y  et al. Alterations in axonal transport motor proteins in sporadic and experimental Parkinson's disease. Brain 135:2058-73 (2012). ICC/IF ; Rat . Read more (PubMed: 22719003) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"