Anti-Dynorphin A antibody (ab40647)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Dynorphin A
  • Specificity
    This antibody shows specificity of: Dynorphin A (1-13) 100%; [Leu5]-Enkephalin 0%; [Met5]-Enkephalin 0% and b-Endorphin (human) 0%.
  • Tested applications
    Suitable for: IHC-P, ELISA, RIA, ICCmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Chimpanzee, Zebrafish, Baboon, Cynomolgus monkey, Macaque monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide:

    YGGFLRRIRPKLK

    , corresponding to amino acids 1-13 of Dynorphin A

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
RIA Use at an assay dependent concentration.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC Use at an assay dependent concentration.

Target

  • Relevance
    Dynorphins are a class of peptides produced by many different populations of neurons, which has some opiate like activity. It is thus classed as an endogenous opioid peptide. The dynorphins, which include dynorphin A, dynorphin B, alpha and beta neoendorphin, and big dynorphin, are all the products of a single gene, 'preprodynorphin'. Dynorphin is produced in many different parts of the brain, including the hypothalamus, the hippocampus and the spinal cord, and has many different physiological actions, depending upon its site of production. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. As such, it may help some individuals against addiction. Blocking dynorphin may help alleviate depression.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • ADCA antibody
    • Beta neoendorphin dynorphin antibody
    • Dyn A17 antibody
    • leu-enkephalin antibody
    • leumorphin antibody
    • neoendorphin-dynorphin-enkephalin prepropeptide antibody
    • PDYN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Proenkephalin B antibody
    • rimorphin antibody
    • SCA23 antibody
    see all

Anti-Dynorphin A antibody images

  • IHC image of ab40647 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40647, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Dynorphin A antibody (ab40647)

ab40647 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call yesterday and for your question about these antibodies. I apologize for the delay getting back to you.

We have a general IHC protocol for FFPE sections that includes a section on antigen retrieval-

http://www...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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