Anti-Dynorphin A antibody (ab82509)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Dynorphin A
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Cow, Pig, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Dynorphin A.

    (Peptide available as ab91634.)

  • Positive control
    • This antibody gave a positive signal in human brain membrane tissue lysate. IHC-P: FFPE mouse dorsal root ganglion.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • Relevance
    Dynorphins are a class of peptides produced by many different populations of neurons, which has some opiate like activity. It is thus classed as an endogenous opioid peptide. The dynorphins, which include dynorphin A, dynorphin B, alpha and beta neoendorphin, and big dynorphin, are all the products of a single gene, 'preprodynorphin'. Dynorphin is produced in many different parts of the brain, including the hypothalamus, the hippocampus and the spinal cord, and has many different physiological actions, depending upon its site of production. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. As such, it may help some individuals against addiction. Blocking dynorphin may help alleviate depression.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • ADCA antibody
    • Beta neoendorphin dynorphin antibody
    • Dyn A17 antibody
    • leu-enkephalin antibody
    • leumorphin antibody
    • neoendorphin-dynorphin-enkephalin prepropeptide antibody
    • PDYN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Proenkephalin B antibody
    • rimorphin antibody
    • SCA23 antibody
    see all

Anti-Dynorphin A antibody images

  • IHC image of Dynorphin A staining in formalin fixed, paraffin embedded mouse dorsal root ganglion tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82509, 10µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-Dynorphin A antibody (ab82509) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa


    Exposure time : 15 minutes
  • ICC/IF image of ab82509 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
  • IHC image of Dynorphin A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82509, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • Anti-Dynorphin A antibody (ab82509) at 1/1000 dilution + Mouse synaptosome from hippocampal stratum lucidum tissue lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa (possible non-specific binding),65 kDa (possible non-specific binding).

    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Dynorphin A antibody (ab82509)

ab82509 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - other (synaptosome from hippocampal stratum lucidum)
Specification
synaptosome from hippocampal stratum lucidum
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 23 2014

Thank you for your call yesterday and for your question about these antibodies. I apologize for the delay getting back to you.

We have a general IHC protocol for FFPE sections that includes a section on antigen retrieval-

http://www...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up