Anti-DYRK1A antibody (ab156818)
Key features and details
- Goat polyclonal to DYRK1A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DYRK1A antibody
See all DYRK1A primary antibodies -
Description
Goat polyclonal to DYRK1A -
Host species
Goat -
Specificity
ab156818 is expected to recognize all reported isoforms (NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1).
ab156818 is not expected to cross-react with DYRK1B. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human DYRK1A aa 35-47 (N terminal) (Cysteine residue).
Sequence:PHSHQYSDRRQPN
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Positive control
- K562 cell line lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab156818 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates. -
Tissue specificity
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. -
Involvement in disease
Defects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain. -
Domain
The polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028). -
Post-translational
modificationsAutophosphorylated on tyrosine residues. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 1859 Human
- Entrez Gene: 13548 Mouse
- Entrez Gene: 25255 Rat
- Omim: 600855 Human
- SwissProt: Q13627 Human
- SwissProt: Q61214 Mouse
- SwissProt: Q63470 Rat
- Unigene: 368240 Human
see all -
Alternative names
- Dual specificity tyrosine phosphorylation regulated kinase antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
see all
Images
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Anti-DYRK1A antibody (ab156818) at 0.5 µg/ml + K562 cell line lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab156818 has been referenced in 1 publication.
- Wang P et al. Dual-specificity tyrosine-phosphorylation regulated kinase 1A Gene Transcription is regulated by Myocyte Enhancer Factor 2D. Sci Rep 7:7240 (2017). PubMed: 28775333