Overview

  • Product nameAnti-DYRK1A antibody
    See all DYRK1A primary antibodies
  • Description
    Rabbit polyclonal to DYRK1A
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human DYRK1A aa 107-136 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q13627

  • Positive control
    • 293 cell line lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThis antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
WB 1/50 - 1/100. Detects a band of approximately 46, 60, 86 kDa (predicted molecular weight: 86 kDa).
ELISA 1/1000.

Target

  • FunctionMay play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates.
  • Tissue specificityUbiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
  • Involvement in diseaseDefects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
    Contains 1 protein kinase domain.
  • Developmental stageExpressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain.
  • DomainThe polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028).
  • Post-translational
    modifications
    Autophosphorylated on tyrosine residues.
  • Cellular localizationNucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity tyrosine phosphorylation regulated kinase antibody
    • Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1A antibody
    • Dual specificity tyrosine-phosphorylation-regulated kinase 1A antibody
    • Dual specificity YAK 1 related kinase antibody
    • Dual specificity YAK1 related kinase antibody
    • Dual specificity YAK1-related kinase antibody
    • DYR1A_HUMAN antibody
    • DYRK 1 antibody
    • DYRK 1A antibody
    • DYRK antibody
    • DYRK1 antibody
    • Dyrk1a antibody
    • DYRKA antibody
    • hMNB antibody
    • HP 86 antibody
    • HP86 antibody
    • Minibrain (Drosophila) homolog antibody
    • Minibrain homolog antibody
    • Minibrain, Drosophila, homolog of antibody
    • MNB antibody
    • MNB protein kinase antibody
    • Mnb protein kinase homolog hp86 antibody
    • MNB protein kinase, Serine/threonine-specific antibody
    • MNB/DYRK protein kinase antibody
    • MNBH antibody
    • MRD7 antibody
    • OTTHUMP00000109090 antibody
    • OTTHUMP00000109091 antibody
    • OTTHUMP00000109094 antibody
    • OTTHUMP00000174799 antibody
    • Protein kinase minibrain homolog antibody
    • Serine/threonine kinase MNB antibody
    see all

Anti-DYRK1A antibody images

  • Anti-DYRK1A antibody (ab71464) at 1/60 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 46 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DYRK1A antibody (ab71464)

This product has been referenced in:
  • Hong JY  et al. Down's-syndrome-related kinase Dyrk1A modulates the p120-catenin-Kaiso trajectory of the Wnt signaling pathway. J Cell Sci 125:561-9 (2012). WB . Read more (PubMed: 22389395) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 16 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization No
Blocking step (agent) for 45 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2009

Thank you for your enquiry. ab16140:The antigen made to generate the antibody is specific to Dyrk1A. That said, we have never actually tested against any other Dyrk protein, but the Dyrk1A sequence differs so greatly from Dyrk1B that it would be hig...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"