Overview

  • Product name
  • Description
    Rabbit polyclonal to Dyrk1B
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 500-550 of Human Dyrk1B (NP_004705.1).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 5-15 µg/mg of lysate.

Target

  • Function
    Dual-specificity kinase which possesses both serine/ threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments.
  • Tissue specificity
    Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AOMS3 antibody
    • Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1B antibody
    • Dual specificity tyrosine-phosphorylation-regulated kinase 1B antibody
    • DYR 1B antibody
    • DYR1A antibody
    • DYR1B antibody
    • DYR1B_HUMAN antibody
    • DYRK 1B antibody
    • Dyrk1b antibody
    • Minibrain related kinase antibody
    • Minibrain-related kinase antibody
    • MIRK antibody
    • Mirk protein kinase antibody
    see all

Images

  • All lanes : Anti-Dyrk1B antibody (ab113968) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa


    Exposure time : 1 minute
  • Detection of Dyrk1B in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113968 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab113968 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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