Overview

  • Product nameAnti-Dyrk1B antibody [EPR5617]
    See all Dyrk1B primary antibodies
  • Description
    Rabbit monoclonal [EPR5617] to Dyrk1B
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Dyrk1B.

  • Positive control
    • HT 1080, Neuro 2a, SW480 and HeLa cell lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-Dyrk1B antibody (Alexa Fluor® 647) [EPR5617] (ab207056)
    Anti-Dyrk1B antibody (Alexa Fluor® 488) [EPR5617] (ab207372)

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionDual-specificity kinase which possesses both serine/ threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments.
    • Tissue specificityHighest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AOMS3 antibody
      • Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B antibody
      • Dual specificity tyrosine phosphorylation regulated kinase 1B antibody
      • Dual specificity tyrosine-phosphorylation-regulated kinase 1B antibody
      • DYR 1B antibody
      • DYR1A antibody
      • DYR1B antibody
      • DYR1B_HUMAN antibody
      • DYRK 1B antibody
      • Dyrk1b antibody
      • Minibrain related kinase antibody
      • Minibrain-related kinase antibody
      • MIRK antibody
      • Mirk protein kinase antibody
      see all

    Anti-Dyrk1B antibody [EPR5617] images

    • All lanes : Anti-Dyrk1B antibody [EPR5617] (ab124960) at 1/1000 dilution

      Lane 1 : HT 1080 cell lysates
      Lane 2 : Neuro 2a cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
    • ab124960, at 1/50 dilution staining Dyrk1B in paraffin-embedded Human brain tissue, by Immunohistochemistry.

    References for Anti-Dyrk1B antibody [EPR5617] (ab124960)

    ab124960 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124960.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"