Purification notesThis antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
1/50 - 1/100.
FunctionRole in the regulation of cellular growth and/or development. Regulates TP53 by phosphorylation on Ser-46 to induce apoptosis in response to DNA damage, functioning downstream of ATM. Inactivates GYS1 by phosphorylation at Ser-641, and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Phosphorylates EIF2B5 at Ser-544, enabling its subsequent phosphorylation and inhibition by GSK3, and may play a more general role in the priming of GSK3 substrates.
Tissue specificityTestis, after the onset of spermatogenesis.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated on serine/threonine residues.
Cellular localizationCytoplasm. Nucleus. Translocates into the nucleus following DNA damage.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling DYRK2 with ab37912 at 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
Western blot - DYRK2 antibody (ab37912)
Anti-DYRK2 antibody (ab37912) at 1/1000 dilution + HEK 293 whole cell lysate at 35 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 60 kDa Observed band size : 60 kDa Additional bands at : 17 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Blocking/Dilution buffer: 5% NFDM/TBST.
References for Anti-DYRK2 antibody (ab37912)
This product has been referenced in:
Maddika S & Chen J Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase. Nat Cell Biol11:409-19 (2009).
Read more (PubMed: 19287380) »