Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Can be blocked with Human Dysadherin peptide (ab22894).

Target

  • FunctionInvolved in down-regulation of E-cadherin which results in reduced cell adhesion. Promotes metastasis.
  • Sequence similaritiesBelongs to the FXYD family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dysad antibody
    • Dysadherin antibody
    • FXYD 5 antibody
    • FXYD domain containing ion transport regulator 5 antibody
    • FXYD domain-containing ion transport regulator 5 antibody
    • Fxyd5 antibody
    • FXYD5_HUMAN antibody
    • FXYD5RIC antibody
    • HSPC 113 antibody
    • HSPC113 antibody
    • IWU 1 antibody
    • IWU1 antibody
    • KCT 1 antibody
    • KCT1 antibody
    • Keratinocytes associated transmembrane protein 1 antibody
    • OIT 2 antibody
    • OIT2 antibody
    • PRO 6241 antibody
    • PRO6241 antibody
    • RIC antibody
    see all

Anti-Dysadherin antibody images

  • ab2248 (0.5 µg/ml) staining Human Spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-Dysadherin antibody (ab2248)

This product has been referenced in:
  • Arystarkhova E  et al. Multiplicity of expression of FXYD proteins in mammalian cells: dynamic exchange of phospholemman and gamma-subunit in response to stress. Am J Physiol Cell Physiol 292:C1179-91 (2007). WB ; Human . Read more (PubMed: 17050615) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (multiple pancreatic tumor cell lines)
Loading amount 30 µg
Specification multiple pancreatic tumor cell lines
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris/MES)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 27°C
Username

Dr. Patrick Kaminker

Verified customer

Submitted Sep 15 2009

I'm sorry to hear you are having problems with ab2248. Thank you for taking the time to fill in the questionnaire, it is very useful for me to understand where the problem might be. I would like to recommend an overnight incubation at 4C with the a...

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To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"