Anti-Dysbindin antibody (ab36646)

Overview

  • Product nameAnti-Dysbindin antibody
    See all Dysbindin primary antibodies
  • Description
    Rabbit polyclonal to Dysbindin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Dysbindin.

    (Peptide available as ab36645.)

  • Positive control
    • ab36646 gives a positive signal in the following whole cell lysates: Brain (Mouse) normal tissue Testis (Mouse) normal tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).

Target

  • FunctionThe BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. May be required for normal dopamine homeostasis in the cerebral cortex, hippocampus, and hypothalamus. Plays a role in the regulation of cell surface exposure of DRD2. Contributes to the regulation of dopamine signaling. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation.
  • Tissue specificityDetected in brain, in neurons and in neuropil. Detected in dentate gyrus and in pyramidal cells of hippocampus CA2 and CA3 (at protein level).
  • Involvement in diseaseDefects in DTNBP1 are the cause of Hermansky-Pudlak syndrome type 7 (HPS7) [MIM:203300]. Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.
  • Sequence similaritiesBelongs to the dysbindin family.
  • Post-translational
    modifications
    Ubiquitinated by TRIM32. Ubiquitination leads to DTNBP1 degradation.
    Phosphorylated by PRKDC.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Endosome membrane. Melanosome membrane. Nucleus. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Endoplasmic reticulum. Detected in neuron cell bodies, axons and dendrites. Detected at synapses, at post-synaptic density, at pre-synaptic vesicle membranes and microtubules. Detected at tubulovesicular elements in the vicinity of the Golgi apparatus and of melanosomes. Occasionally detected at the membrane of pigmented melanosomes in cultured melanoma cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • DTBP1_HUMAN antibody
    • DTNBP1 antibody
    • Dysbindin antibody
    • Dysbindin-1 antibody
    • Dystrobrevin binding protein 1 antibody
    • Dystrobrevin-binding protein 1 antibody
    • Hermansky Pudlak syndrome 7 protein antibody
    • Hermansky-Pudlak syndrome 7 protein antibody
    • HPS7 antibody
    • HPS7 protein antibody
    see all

Anti-Dysbindin antibody images

  • All lanes : Anti-Dysbindin antibody (ab36646) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa,35 kDa (possible isoform),80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Dysbindin antibody (ab36646)

ab36646 has not yet been referenced specifically in any publications.

Product Wall

Thank you for you patience in this matter
I have been in contact with the lab about your inquires and there response is below:
"the immunogen ************** is present in both isoforms 1 and 2 so it should recognize both of these. We don...

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Thank you for your reply and your patience in this matter.

I am in contact with the lab to get the QC data for this antibody. As soon as I get that information, I will forward it on to you.

In the meantime please let me know if ther...

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Thank you for contacting Abcam.

According to our data ab36646 detects a strong band at 33 kDa in mouse testis whole cell lysate. This band is thought to correspond to isoform 2 of the protein (SwissProt data). The sequence of the peptide used...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

Submitted May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"