Anti-dystrophia myotonica containing WD repeat motif antibody (ab85932)

Overview

  • Product name
    Anti-dystrophia myotonica containing WD repeat motif antibody
    See all dystrophia myotonica containing WD repeat motif primary antibodies
  • Description
    Rabbit polyclonal to dystrophia myotonica containing WD repeat motif
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 609 and 659 of human dystrophia myotonica containing WD repeat motif (NP_004934.1).

  • Positive control
    • HeLa and 293T whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab85932 was affinity purified using an epitope specific to dystrophia myotonica containing WD repeat motif immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Could have a regulatory function in meiosis.
    • Tissue specificity
      Strongest expression in brain, liver, and testis. Also expressed in kidney and spleen.
    • Sequence similarities
      Contains 5 WD repeats.
    • Information by UniProt
    • Database links
    • Alternative names
      • D19S593E antibody
      • DM 9 antibody
      • DM9 antibody
      • DMR N9 antibody
      • DMR N9 protein antibody
      • DMRN 9 antibody
      • DMRN9 antibody
      • DMWD antibody
      • DMWD_HUMAN antibody
      • Dystrophia myotonica containing WD repeat motif antibody
      • Dystrophia myotonica containing WD repeat motif protein antibody
      • Dystrophia myotonica WD repeat containing protein antibody
      • Dystrophia myotonica WD repeat-containing protein antibody
      • Dystrophia myotonica-containing WD repeat motif protein antibody
      • Gene59 antibody
      • Protein 59 antibody
      • Protein DMR-N9 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling dystrophia myotonica containing WD repeat motif with ab85932 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-dystrophia myotonica containing WD repeat motif antibody (ab85932) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 69 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    References

    ab85932 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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