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Synthetic peptide within Human Dystrophin (C terminal). The exact sequence is proprietary.
Our Abpromise guarantee covers the use of ab15277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||1/400. Use with acetone-fixed tissues.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 427 kDa.|
|IHC - Wholemount||Use at an assay dependent concentration. PubMed: 24852842|
Muscle stem cells (from normal mouse) were injected into the gastric muscle of an MDX mouse. Dystrophin staining: primary antibody ab15277 and secondary antibody is donkey anti-rabbit Alexa 594.
This image was kindly supplied as part of the review submitted by Jessica Tebbets.
ab15277 staining dog muscle cells by ICC/IF. Cells were methanol fixed and incubated with ab15277, diluted 1/200, for 12 hours at 4°C. A FITC conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
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