Recombinant Anti-Dystrophin antibody [EPR21189] (ab218198)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21189] to Dystrophin
- Suitable for: IHC-P, WB, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Dystrophin antibody [EPR21189]
See all Dystrophin primary antibodies -
Description
Rabbit monoclonal [EPR21189] to Dystrophin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse cardiac muscle and skeletal muscle tissue; rat colon tissue. WB: Mouse and rat heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21189 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 427 kDa.
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IHC-Fr | (1) |
1/50.
Positive membrane staining of cardiomyocytes on mouse and Rat heart tissue section (PMID: 26430202). |
Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 427 kDa. |
IHC-Fr
1/50. Positive membrane staining of cardiomyocytes on mouse and Rat heart tissue section (PMID: 26430202). |
Target
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Function
Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. -
Tissue specificity
Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Isoform 5 is expressed in heart, brain, liver, testis and hepatoma cells. Most tissues contain transcripts of multiple isoforms, however only isoform 5 is detected in heart and liver. -
Involvement in disease
Defects in DMD are the cause of Duchenne muscular dystrophy (DMD) [MIM:310200]. DMD is the most common form of muscular dystrophy; a sex-linked recessive disorder. It typically presents in boys aged 3 to 7 year as proximal muscle weakness causing waddling gait, toe-walking, lordosis, frequent falls, and difficulty in standing up and climbing up stairs. The pelvic girdle is affected first, then the shoulder girdle. Progression is steady and most patients are confined to a wheelchair by age of 10 or 12. Flexion contractures and scoliosis ultimately occur. About 50% of patients have a lower IQ than their genetic expectations would suggest. There is no treatment.
Defects in DMD are the cause of Becker muscular dystrophy (BMD) [MIM:300376]. BMD resembles DMD in hereditary and clinical features but is later in onset and more benign.
Defects in DMD are a cause of cardiomyopathy dilated X-linked type 3B (CMD3B) [MIM:302045]; also known as X-linked dilated cardiomyopathy (XLCM). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Contains 2 CH (calponin-homology) domains.
Contains 22 spectrin repeats.
Contains 1 WW domain.
Contains 1 ZZ-type zinc finger. -
Cellular localization
Cell membrane > sarcolemma. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 13405 Mouse
- Entrez Gene: 24907 Rat
- SwissProt: P11531 Mouse
- SwissProt: P11530 Rat
- Unigene: 275608 Mouse
- Unigene: 416750 Mouse
- Unigene: 10307 Rat
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Alternative names
- BMD antibody
- CMD3B antibody
- DMD antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded (A) Skeletal muscle tissue from wild-type C57BL/6JGpt mice (B) Skeletal muscle tissue from DMD knockout mice staining with ab218198 at 1/5000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on (A) Skeletal muscle tissue from wild-type C57BL/6JGpt mice and no staining on (B) Skeletal muscle tissue from DMD knockout mice.The section was incubated with ab218198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and DMD-KO homozygous mice (Strain ID: T003035).The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and DMD-KO homozygous mice (Strain ID: T003035).
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Immunohistochemical analysis of paraffin-embedded (A) Cardiac muscle tissue from wild-type C57BL/6JGpt mice (B) Cardiac muscle tissue from DMD knockout mice staining with ab218198 at 1/5000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on (A) Cardiac muscle tissue from wild-type C57BL/6JGpt mice and no staining on (B) Cardiac muscle tissue from DMD knockout mice. The section was incubated with ab218198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and DMD-KO homozygous mice (Strain ID: T003035). -
All lanes : Anti-Dystrophin antibody [EPR21189] (ab218198) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 427 kDa
Observed band size: 425 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-2: 180 seconds; Lane 3-4: 81 seconds.
Negative control: mouse liver and rat liver.
In Western blot, anti-Vinculin antibody (ab129002) staining at 1/10, 000 dilution.
Samples are non-boiled as boiling may cause protein aggregation. -
Immunohistochemical (Frozen sections) analysis of rat heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.
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Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in smooth muscle of rat colon (PMID: 18806224) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical (Frozen sections) analysis of mouse heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse skeletal muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse cardiac muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab218198 has been referenced in 1 publication.
- Meyers TA et al. DMD carrier model with mosaic dystrophin expression in the heart reveals complex vulnerability to myocardial injury. Hum Mol Genet 29:944-954 (2020). PubMed: 31976522