Anti-DZIP3 antibody (ab56657)

Overview

  • Product nameAnti-DZIP3 antibody
    See all DZIP3 primary antibodies
  • Description
    Mouse monoclonal to DZIP3
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: PSAGLRSDPS IMNWERITDR LKTAFPQQTR KELTDFLRKL KDAYGKSLSE LTFDEIVCKI SQFIDPKKSQ SQGKSVSNVN CVSPSHSPSQ PDAAQPPKPA , corresponding to amino acids 1021-1121 of Human DZIP3

Properties

Applications

Our Abpromise guarantee covers the use of ab56657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceE3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.
    • Cellular localizationCytoplasm
    • Database links
    • Alternative names
      • DAZ-interacting protein 3 antibody
      • E3 ubiquitin-protein ligase DZIP3 antibody
      • hRUL138 antibody
      • KIAA0675 antibody
      • RNA-binding ubiquitin ligase of 138 kDa antibody
      see all

    Anti-DZIP3 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56657 DZIP3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-DZIP3 antibody (ab56657)

    ab56657 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HEK293)
    Total protein in input 10 µg
    Specification HEK293
    Immuno-precipitation step Other - Dynabeads
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2012

    Thank you for your reply.

    We cannot extend the expiration of these discount codes but I generated a new code for you. Once you submit the Abreview for how ab56657 works in IP, you can use the code below to claim your free primary antibody. Read More

    Thank you for contacting Abcam.

    Please check the expiration date on the testing discount that was sent to you and see if it is still valid. If the code has expired please let me know and I will see if it is possible to extend the expiration d...

    Read More

    DISCOUNT CODE: *******Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab56657 in Immunoprecipitation. This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please ...

    Read More

    Thank you for your interest in testing ab56657 in IHC on mouse tissue. I have checked the data sheet for anti-DZIP3 and it has not been tested in IP and so if you would rather have the testing discount for testing ab56657 in IP rather than IHC, that wo...

    Read More

    Thank you for contacting Abcam about ab56657. . Unfortunately we do not offer sample sizes of our antibodies, but what I can offer is our testing discount program, the details of which are below.  If this is something that you woul...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application CLIP
    Sample Human Cell lysate - whole cell (HEK 293T)
    Specification HEK 293T
    Type iCLIP
    Irradiation Energy Level (mJ/cm2): 150
    Wavelength (nm): 254
    Positive control hnRNP C antibody (sc-15386) bound protein G dynabeads 0.1 mg/ml (antibody/beads).
    Negative control Protein G dynabeads without antibody bound.
    Immuno-precipitation step Other - Protein A dynabeads
    Username

    Abcam user community

    Verified customer

    Submitted Aug 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"