• Product nameAnti-E Cadherin antibody
    See all E Cadherin primary antibodies
  • Description
    Rabbit polyclonal to E Cadherin
  • SpecificityThis antibody reacts specifically with 89-91 kDa of rat E Cadherin protein.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WB, Flow Cyt, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide derived from the N terminal region of rat E Cadherin.



Our Abpromise guarantee covers the use of ab53226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50. PubMed: 19636430
ICC/IF Use at an assay dependent concentration. PubMed: 23516613
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 97 kDa.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration.


  • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificityNon-neural epithelial tissues.
  • Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similaritiesContains 5 cadherin domains.
  • Post-translational
    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arc 1 antibody
    • CADH1_HUMAN antibody
    • Cadherin 1 antibody
    • cadherin 1 type 1 E-cadherin antibody
    • Cadherin1 antibody
    • CAM 120/80 antibody
    • CD 324 antibody
    • CD324 antibody
    • CD324 antigen antibody
    • cdh1 antibody
    • CDHE antibody
    • E-Cad/CTF3 antibody
    • E-cadherin antibody
    • ECAD antibody
    • Epithelial cadherin antibody
    • epithelial calcium dependant adhesion protein antibody
    • LCAM antibody
    • Liver cell adhesion molecule antibody
    • UVO antibody
    • Uvomorulin antibody
    see all

Anti-E Cadherin antibody images

  • ab53226 staining E Cadherin in rat mammary tumor tissue by Immunohistochemistry (Frozen sections).
    Staining for E Cadherin was performed on frozen sections of tumor tissue using ab53226 diluted 1/50 and incubated for 2 hours at room temperature. Staining was done using the EnVision-labeled polymer method. The antigens were localized by the diaminobenzidine tetrachloride peroxidase reaction, and the slides were counterstained with Mayer's hematoxylin.
  • Flow cytometry analysis of rat primary hepatocytes, staining E Cadherin with ab53226.

    Cells were cultured in Cells were harvested by gentle scraping in ice cold PBS with 10% FCS and 1% sodium azide. The sample was incubated with the primary antibody (1/50 in 3% BSA) for 1 hour at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    Gating Strategy: High FSC/SSC

    See Abreview

References for Anti-E Cadherin antibody (ab53226)

This product has been referenced in:
  • Xing Y  et al. Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-ß2. Int J Mol Med N/A:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27315599) »
  • Liu L  et al. SALL4 as an Epithelial-Mesenchymal Transition and Drug Resistance Inducer through the Regulation of c-Myc in Endometrial Cancer. PLoS One 10:e0138515 (2015). WB ; Human . Read more (PubMed: 26407074) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Primary hepatocytes)
Specification Primary hepatocytes
Preparation Cell harvesting/tissue preparation method: Gentle scraping in ice cold sample buffer
Sample buffer: PBS with 10% FCS and 1% Sodium Azide
Fixation None
Permeabilization No
Gating Strategy High FSC/SSC

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Submitted Oct 18 2012

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