Overview

  • Product nameAnti-E Cadherin antibody [DECMA-1]
    See all E Cadherin primary antibodies
  • Description
    Rat monoclonal [DECMA-1] to E Cadherin
  • SpecificitySome of our customers have had good results using ab11512 in human samples, particularly in ICC. In our hands however this product does not work in human samples. Please contact Scientific Support for further information or queries.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Dog
    Predicted to work with: Cow
  • Immunogen

    Mouse embryonal carcinoma cell line PCC4 Aza RI.

  • General notes

    Alternative versions available:

    Anti-E Cadherin antibody (FITC) [DECMA-1] (ab150004)

Properties

Applications

Our Abpromise guarantee covers the use of ab11512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 20521328

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. PubMed: 19586906
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificityNon-neural epithelial tissues.
  • Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similaritiesContains 5 cadherin domains.
  • Post-translational
    modifications
    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arc 1 antibody
    • CADH1_HUMAN antibody
    • Cadherin 1 antibody
    • cadherin 1 type 1 E-cadherin antibody
    • Cadherin1 antibody
    • CAM 120/80 antibody
    • CD 324 antibody
    • CD324 antibody
    • CD324 antigen antibody
    • cdh1 antibody
    • CDHE antibody
    • E-Cad/CTF3 antibody
    • E-cadherin antibody
    • ECAD antibody
    • Epithelial cadherin antibody
    • epithelial calcium dependant adhesion protein antibody
    • LCAM antibody
    • Liver cell adhesion molecule antibody
    • UVO antibody
    • Uvomorulin antibody
    see all

Anti-E Cadherin antibody [DECMA-1] images

  • ab11512 staining E cadherin in SW480 cells treated with Src Kinase Inhibitor I (SKI-1) (ab120839), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of Src Kinase Inhibitor I (SKI-1), as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120839 (Src Kinase Inhibitor I (SKI-1)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab11512 E Cadherin staining canine kidney (MDCK) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 37°C. Samples were incubated with primary antibody, 1/100, in blocking buffer for 1 hour at 37°C. An undiluted Cy3®-conjugated Donkey polyclonal to rat IgG was used as secondary antibody.

    See Abreview

  • ab11512 staining E cadherin in SW480 cells treated with PP3 (ab120617), by ICC/IF. No change of E cadherin expression with increased concentration of PP3 is observed, as described in literature, since PP3 is the negative control for PP2.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120617 (PP3) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ICC/IF image of ab11512 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11512 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Formaldehyde fixed (4%) (10 min) MCF-7 cells

  • ab11512 staining E-Cadherin in SW480 cells treated with PP2 (ab120308), by ICC/IF. Increase in E-cadherin expression correlates with increased concentration of PP2, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120308 (PP2) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab96971) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab11512 staining E Cadherin in Mouse embryonic (E14.5) lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 3 hours at 4°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 14 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab11512 staining E cadherin in SW480 cells treated with PP1 (ab120859), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of PP1, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120859 (PP1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab11512 at 1/500 staining dog kidney cells by ICC/IF. The cells were methanol fixed and blocked with BSA before incubation with the antibody for 18 hours at 4°C. An Alexa Fluor ® 555 conjugated goat anti-rat IgG was used as the secondary.

    See Abreview

References for Anti-E Cadherin antibody [DECMA-1] (ab11512)

This product has been referenced in:
  • Wei SC  et al. Matrix stiffness drives epithelial-mesenchymal transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway. Nat Cell Biol 17:678-88 (2015). Human . Read more (PubMed: 25893917) »
  • Kopp F  et al. Sequential Salinomycin Treatment Results in Resistance Formation through Clonal Selection of Epithelial-Like Tumor Cells. Transl Oncol 7:702-11 (2014). WB, IHC . Read more (PubMed: 25500079) »

See all 22 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, postnatal day 6
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 11 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung)
Permeabilization Yes - .1% SDS
Specification Lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 20 2015

We have tested the conjugated form of this antibody in flow cytometry, Anti-E Cadherin antibody [DECMA-1] (FITC) (ab150004).

The protocol we used is the following:

The cells were fixed with 80% methanol (5 min) and then permeabilize...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 16 2012

Thank you for your phone call.

I am sorry to hear that both antibodies do not work as expected.


As for ab92539, I am sending you - as we discussed - the testing discount code for having tried it in IHC-Fr. After submitting you...

Read More

Thank you for contacting us. FAM is a fluorochrome with an excitation of 494 and emission wavelength 519. We have tested using secondaries conjugated to Alexa594, Alexa555, and Cy3 with good results.

I hope this information is helpful to you. ...

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Vielen Dank für Ihre Anfrage.

Das Immunogen für ab11512 (Anti-E Cadherin antibody [DECMA-1])n was eine Zellpräparation von einer murinen embryonalen Carcimomzelllinie.

Unseres Wissens wurde das genaue Epitop das der A...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse embryonic lung (E14.5))
Specification Mouse embryonic lung (E14.5)
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted May 28 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse embryonic lung (E14.5))
Specification Mouse embryonic lung (E14.5)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH=6)
Permeabilization No
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 26 2012

Thank you for contacting us.
I have checked the homologies for the mouse and human protein with the zebrafish sequence.
human - zebrafish = 53% (C-terminus aligns better than the rest of the protein)
mouse - zebrafish = 81%
The anti...

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1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"