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Synthetic peptide corresponding to residues in the 5th domain of human E-cadherin
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Alternative versions available:
Our Abpromise guarantee covers the use of ab40772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24915897|
Permeabilisation is unnecessary as the immunogen is in an extracellular domain.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Predicted molecular weight: 120 kDa.|
ab40772 showing positive staining in human colonic adenocarcinoma tissue.
Blocking and diluting buffer and concentration 5% NFDM/TBST.
The full-length of E-cadherin is 120 kDa. The other bands are due to proteolytic cleavages in different Cadherin domains. (Ref: PMID: 14695147)
ICC/IF image of ab40772 stained human MCF7 cells. The cells were fixed in Paraformaldehyde, before incubation in 0.1% BSA + 1% goat serum in PBS as a blocking agent for 30 mins at 22oC. The cells were then incubated with the antibody (ab40772, 1:100 dilution) for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
ab40772 showing positive staining in human lung adenocarcinoma tissue.
ab40772 showing positive staining in human breast carcinoma tissue.
ab40772 showing positive staining in human papillary carcinoma of thyroid gland tissue.
ab40772 showing positive staining in human transitional cell carcinoma of kidney tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"