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Breast tumor cell line MCF-7
Our Abpromise guarantee covers the use of ab1416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19244118|
ab1416 staining E cadherin in Human renal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.01% BSA) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody (1/500).
Breast cancer stained with E Cadherin mouse antibody ab1416
Blocking buffer: 3% milk
This image is courtesy of an Abreview submitted by Dr Alban Gaultier
ab1416 staining E Cadherin in the Human NSCLC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton in PBS and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 0.3% Triton, 5% Goat Serum in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat polyclonal was used as the secondary antibody (1/500).
ab1416 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.
Immunofluorescence of the frozen tissue section showed that the anti-E cadherin antibody localised its antigen throughout the human mucosal epithelium. No background appeared in the connective tissue.
This image was kindly supplied as part of the review submitted by Marko Nykanen.
ab1416 staining E Cadherin in Human Renal Proximal Tubular cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton ×100 and blocking with 1% BSA was done for 30 minutes at 210C. Samples were incubated with primary antibody (1/50: in 0.1 % BSA in PBS) for 24 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"