Overview

  • Product nameAnti-E Cadherin antibody [HECD-1]
    See all E Cadherin primary antibodies
  • Description
    Mouse monoclonal [HECD-1] to E Cadherin
  • Tested applicationsSuitable for: Flow Cyt, ICC, IP, IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Breast tumor cell line MCF-7

  • Positive control
    • Breast Cancer

Properties

Applications

Our Abpromise guarantee covers the use of ab1416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 19244118
WB 1/50.

Target

  • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificityNon-neural epithelial tissues.
  • Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similaritiesContains 5 cadherin domains.
  • Post-translational
    modifications
    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arc 1 antibody
    • CADH1_HUMAN antibody
    • Cadherin 1 antibody
    • cadherin 1 type 1 E-cadherin antibody
    • Cadherin1 antibody
    • CAM 120/80 antibody
    • CD 324 antibody
    • CD324 antibody
    • CD324 antigen antibody
    • cdh1 antibody
    • CDHE antibody
    • E-Cad/CTF3 antibody
    • E-cadherin antibody
    • ECAD antibody
    • Epithelial cadherin antibody
    • epithelial calcium dependant adhesion protein antibody
    • LCAM antibody
    • Liver cell adhesion molecule antibody
    • UVO antibody
    • Uvomorulin antibody
    see all

Anti-E Cadherin antibody [HECD-1] images

  • ab1416 staining E cadherin in Human renal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.01% BSA) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • Breast cancer stained with E Cadherin mouse antibody ab1416

  • Lanes 1 - 2 : Anti-E Cadherin antibody [HECD-1] (ab1416) at 1/50 dilution
    Lanes 3 - 4 : Anti-GAPDH at 1 µg/ml

    Lane 1 : MDA-MB-231 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : MDA-MB-231 cell lysate
    Lane 4 : MCF-7 cell lysate

    Blocking buffer: 3% milk

  • Overlay histogram showing MCF7 cells stained with ab1416 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1416, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab1416 staining E Cadherin in Human nasal epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed paraformaldehyde and permeabilized with Triton X-100 0.1%. Samples were incubated with primary antibody (1/50) for 18 hours at 4°C. A Cy2®-conjugated Horse anti-mouse monoclonal(1/100) was used as the secondary antibody.

    See Abreview

  • ab1416 staining E Cadherin in human normal skin tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone. Samples were blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes at 21°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

    See Abreview

  • All lanes : Anti-E Cadherin antibody [HECD-1] (ab1416) at 1/1000 dilution

    Lane 1 : MDA-MB-468 cells whole cell lysate
    Lane 2 : MDA-MB-468 cells whole cell lysate

    Lysates/proteins at 80 µg/ml per lane.

    Secondary
    HRP-conjugated polyclonal donkey anti-mouse IgG

    Performed under reducing conditions.

    Observed band size : 110 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Dr Alban Gaultier

    See Abreview

  • ab1416 staining E Cadherin in the Human NSCLC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton in PBS and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 0.3% Triton, 5% Goat Serum in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • ab1416 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

    See Abreview

  • ab1416 staining E Cadherin in Human Renal Proximal Tubular cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton ×100 and blocking with 1% BSA was done for 30 minutes at 210C. Samples were incubated with primary antibody (1/50: in 0.1 % BSA in PBS) for 24 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.

    See Abreview

References for Anti-E Cadherin antibody [HECD-1] (ab1416)

This product has been referenced in:
  • Peng C  et al. Norcantharidin Suppresses Colon Cancer Cell Epithelial-Mesenchymal Transition by Inhibiting the avß6-ERK-Ets1 Signaling Pathway. Sci Rep 6:20500 (2016). Read more (PubMed: 26846153) »
  • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). Read more (PubMed: 27444869) »

See all 51 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Cow Tissue sections (Fetal Heart)
Specification Fetal Heart
Permeabilization No
Fixative Formaldehyde
Username

Dr. Juliana Shimara Pires Ferrão

Verified customer

Submitted Nov 26 2014

Thank you for sending the image. It may well be that the vial you have received was a bad vial which is why the antibody is not detecting the target although it is very strictly qc tested. If you are interested in trying the same antibody I can sen...

Read More
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Human Cell (Renal tubular epithelial cells)
Specification Renal tubular epithelial cells
Permeabilization Yes - Triton X100, 0.5 %
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 19 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human NSCLC Cell Line)
Specification Human NSCLC Cell Line
Permeabilization Yes - 0.3% Triton in PBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation Methanol
Permeabilization No
Sample Human Cell (A431 cells)
Specification A431 cells
Gating Strategy no gate
Preparation Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
Sample buffer: PBS/10% human IgG
Username

Abcam user community

Verified customer

Submitted Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (nasal epithelial cells)
Specification nasal epithelial cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100 0.1%
Username

Mr. Michael Könnecke

Verified customer

Submitted Feb 13 2013

Muchas gracias por enviarnos el cuestionario relleno con la información del anticuerpo.

Siento que el anticuerpo no haya dado los resultados esperados en ninguna de las técnicas utilizadas, y espero poder ayudar a su optimizaci&...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW480 cells, HCT116 cells)
Loading amount 100000 cells
Specification SW480 cells, HCT116 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Bronchial epitheliel cells from lung)
Loading amount 25 µg
Specification Human Bronchial epitheliel cells from lung
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Dec 19 2012

Thank you for contacting us.

A clone number is given to an antibody produced by a single clone of hybridoma cells. Each hybridoma cell clone produces one single pure homogeneous type of antibody. The term "monoclonal" pertains to a ...

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