Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Renal Proximal Tubular cells)
Specification
Human Renal Proximal Tubular cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1 % BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Username

Dr. Robert Jenkins

Verified customer

Submitted Jun 03 2009

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