Flow Cytometry abreview for Anti-E Cadherin antibody [HECD-1]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
Methanol
Permeabilization
No
Sample
Human Cell (A431 cells)
Specification
A431 cells
Gating Strategy
no gate
Preparation
Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
Sample buffer: PBS/10% human IgG

Other product details

Incubation time
45 minute(s) · Temperature: 4°C · Diluent: Diluent: PBS/10% human IgG
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100

Additional data

Additional Notes
1 = isotype control; 2 = A431 cells.
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Submitted Jul 16 2013

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